CST, 9043S, MAG (D4G3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAG. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MAG (D4G3) Rabbit Monoclonal Antibody (CST #9043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51157 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity MAG (D4G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAG protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg605 of human MAG protein. Background Myelin-associated glycoprotein (MAG), which contains five immunoglobulin-like domains, is a highly glycosylated protein (1). MAG is a component of all myelinated internodes, whether formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS) (2), and has several functions. A known function of MAG is its inhibition of axonal regeneration after injury. It inhibits axonal outgrowth from adult dorsal root ganglion and in postnatal cerebellar, retinal, spinal, hippocampal, and superior cervical ganglion neurons (3). Interaction between MAG and several other molecules on the innermost wrap of myelin and complementary receptors on the opposing axon surface are required for long-term axon stability. Without MAG, myelin is still expressed, but long-term axon degeneration and altered axon cytoskeleton structure can be seen (4). Alternate Names GMA; MAG; myelin associated glycoprotein; Myelin-associated glycoprotein; S-MAG; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; Siglec-4a; SIGLEC4A; SPG75 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG