CST, 90470T, HCAR2/GPR109A (F6D3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPR109A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HCAR2/GPR109A (F6D3M) Rabbit Monoclonal Antibody (CST #90470) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HCAR2/GPR109A (F6D3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total HCAR2/GPR109A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HCAR2/GPR109A protein. Background Hydroxycarboxylic acid receptor 2 (HCAR2), also known as G protein-coupled receptor 109A (GPR109A), is widely expressed in white and brown adipose tissue, keratinocytes, and various immune cells, including monocytes, macrophages, neutrophils, dendritic cells, and microglia (1,2). HCAR2/GPR109A has three significant ligands: nicotinic acid (NA), sodium butyrate (SB), and β-hydroxybutyric acid (BHBA) (2,3). HCAR2/GPR109A activation triggers robust anti-inflammatory effects, leading to anti-atherogenic properties and displaying a role as a potential therapeutic target for treatment of many inflammatory diseases, including neuroinflammatory and/or neurodegenerative diseases such as those affecting the retina and Alzheimer's disease (2,4,5). Alternate Names G protein-coupled receptor 109A; G protein-coupled receptor HM74a; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; G109A; GPR109A; HCA2; HCAR2; HM74A; HM74b; hydroxy-carboxylic acid receptor 2; Hydroxycarboxylic acid receptor 2; Niacin receptor 1; NIACR1; Nicotinic acid receptor; Puma-g; PUMAG Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG