CST, 9047S, Podoplanin (D9D7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Podoplanin. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, Podoplanin (D9D7) Rabbit Monoclonal Antibody (CST #9047) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Flow Cytometry (Live): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 17758 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity Podoplanin (D9D7) Rabbit Monoclonal Antibody recognizes endogenous levels of total podoplanin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp31 of human podoplanin protein. Background Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9). Alternate Names 29kDa cytosolic podoplanin intracellular domain; Aggrus; Glycoprotein 36; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS8; PA2.26; PA2.26 antigen; PDPN; PICD; Podoplanin; T1-alpha; T1A; T1A-2; T1A2; TI1A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG