CST, 90488S, GPC2 (CT3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GPC2. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, GPC2 (CT3) Mouse Monoclonal Antibody (CST #90488) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live) Specificity / Sensitivity GPC2 (CT3) Mouse Monoclonal Antibody recognizes endogenous levels of total GPC2 protein. This antibody reacts with the core GPC2 protein and glycanated forms of GPC2 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with other GPC family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GPC2 protein. Background Glypican 2 (GPC2) is a heparan sulfate proteoglycan that is linked to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage (1). Unlike other members of the glypican family, GPC2 is expressed in the developing nervous system, where it is thought to function as a signaling co-receptor to promote neuronal growth and axon guidance (2,3). GPC2 is considered to be an oncofetal antigen as it is highly expressed in the developing nervous system and then largely silenced in adult, normal tissues. Research studies have shown, however, that GPC2 is highly overexpressed in a high percentage of human neuroblastomas, medulloblastomas, and retinoblastomas. Given the restricted expression pattern of GPC2 in healthy, postnatal tissues, there is significant interest in targeting this antigen using a variety of immunotherapeutic approaches (4-6). Alternate Names cerebroglycan; cerebroglycan proteoglycan; DKFZp547M109; FLJ38962; glypican 2; glypican proteoglycan 2, cerebroglycan proteoglycan; Glypican-2; GPC2; Secreted glypican-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55, 80-300 Source/Isotype: Mouse IgG1 kappa