CST, 90520S, Glut2 (F6X2X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Glut2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Glut2 (F6X2X) Rabbit Monoclonal Antibody (CST #90520) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Glut2 (F6X2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Glut2 protein. This antibody does not cross-react with Glut1, Glut3, Glut4, or Glut5 protein. This antibody detects a 35 kDa and a 160 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Glut2 protein. Background Glucose transporter 2 (Glut2), also known as SLC2A2, is a facilitated hexose transporter. It transports glucose, fructose, and galactose (1-3). Glut2 is expressed in various tissues, such as liver, small intestine, kidney, pancreatic β cells, and central nervous system (1,4). In addition, Glut2 is a marker of mature pancreatic β cells and is required for glucose-stimulated insulin secretion there (1,5). Furthermore, a mutation on human Glut2 protein expressed in oocytes where Val197 is changed to isoleucine was shown to abolish its transport activity (6). Alternate Names Glucose transporter type 2, liver; GLUT-2; GLUT2; GTR2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2 member 2; Solute carrier family 2, facilitated glucose transporter member 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-80 Source/Isotype: Rabbit IgG