CST, 90551T, Tollip (F4Y5L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tollip. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Tollip (F4Y5L) Rabbit Monoclonal Antibody (CST #90551) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Tollip (F4Y5L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tollip protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human Tollip protein. Background Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-interacting protein (Tollip) is an adaptor protein discovered to be associated with the IRAK complex and recruited to IL-1R following IL-1 stimulation (4). Overexpression of Tollip results in impaired NF-κB signaling (4). Tollip also directly associates with TLR2 and TLR4, inhibiting TLR-mediated signaling by inhibiting IRAK (5). Studies of Tollip-deficient mice suggest that it plays a role in regulating inflammatory cytokine responses to IL-1 and LPS (6). Alternate Names adapter protein; FLJ33531; IL-1RAcPIP; TOLIP; toll interacting protein; Toll-interacting protein; TOLLIP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG