CST, 9066S, TCF1/TCF7 (C63D9) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate)

Monoclonal Antibody for studying TCF7. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, TCF1/TCF7 (C63D9) Rabbit Monoclonal Antibody (Pacific Blue^™ Conjugate) (CST #9066) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TCF1/TCF7 (C63D9) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate) detects endogenous levels of total TCF1/TCF7 protein. This antibody conjugate does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro96 of human TCF1/TCF7 protein. Background LEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1/TCF7), TCF3/TCF7L1, and TCF4/TCF7L2 (1). LEF1 and TCF1/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). TCF1/TCF7 has several isoforms due to alternative splicing and transcription from an alternative promoter. The isoforms generated by the alternative promoter do not contain the amino-terminal β-catenin binding domain and therefore may function in a dominant negative manner (6). TCF1/TCF7 displays dynamic expression both in the total amount and the type of isoforms expressed in T cells during development and differentiation (7). Alternate Names FLJ36364; MGC47735; T-cell factor 1; T-cell-factor-7; T-cell-specific transcription factor 1; TCF-1; TCF-7; TCF1; TCF7; Transcription factor 7; transcription factor 7 (T-cell specific, HMG-box); transcription factor-7, T-cell specific Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG