CST, 90673T, EphA1 (D6V7I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EphA1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, EphA1 (D6V7I) Rabbit Monoclonal Antibody (CST #90673) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EphA1 (D6V7I) Rabbit Monoclonal Antibody recognizes endogenous levels of total EphA1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the extracellular domain of human EphA1 protein. Background The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). The EphA1 receptor preferentially binds ephrin-A1 as a ligand (6). This ligand-receptor interaction stimulates EphA1 signaling and regulates cell morphology and motility through the ILK-RhoA-ROCK pathway (7). The gene has been associated with late-onset Alzheimer's diseases (8,9). The role of EphA1 in cancer development falls into two opposite categories. In some type of cancer such as prostate, gastric and liver cancer, high expression of EphA1 associates with cancer metastasis and invasion (10-12). For other types of cancers, such as colon cancer and nonmelanoma skin cancer, downregulation of the protein correlates with cancer development (11,12). The bidirectional signaling modulation of Ephrin-Ephrin receptor interaction might contribute this paradox phenomena (13). Alternate Names EPH; EPH receptor A1; EPH tyrosine kinase; eph tyrosine kinase 1; EPHA1; ephrin receptor EphA1; Ephrin type-A receptor 1; EPHT; EPHT1; erythropoietin-producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; hEpha1; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG