CST, 9067S, RBAP46/RBAP48 (D4F8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBBP7/RBBP4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RBAP46/RBAP48 (D4F8) Rabbit Monoclonal Antibody (CST #9067) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RBAP46/RBAP48 (D4F8) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBAP46 and RBAP48 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RBAP48 protein. Background Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3). Alternate Names CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; G1/S transition control protein-binding protein RbAp46; Histone acetyltransferase type B subunit 2; Histone-binding protein RBBP4; Histone-binding protein RBBP7; lin-53; MGC138867; MGC138868; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP46; Nucleosome-remodeling factor subunit RBAP48; NURF55; RB binding protein 4, chromatin remodeling factor; RB binding protein 7, chromatin remodeling factor; RBAP46; RBAP48; RBBP-4; RBBP-7; RBBP4; RBBP7; retinoblastoma binding protein 4; retinoblastoma binding protein 7; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein 7; Retinoblastoma-binding protein p46; Retinoblastoma-binding protein p48; retinoblastoma-binding protein RbAp46 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG