CST, 90694T, CD64/Fc gammaRI (E2O4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcGR1A mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD64/Fc gammaRI (E2O4L) Rabbit Monoclonal Antibody (CST #90694) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD64/Fc gammaRI (E2O4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD64/FcγRI protein. This antibody does not cross-react with mouse FcγRIV, FcγRIIA, or FcγRIIB. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile256 of mouse CD64/FcγRI protein. Background CD64/FcγRI (high affinity IgG Fc gamma RI) is an activating Fcγ receptor in the immunoglobulin superfamily, predominantly found on macrophages and monocytes (1-4). CD64 has a high affinity for the antibody-constant region of IgG, with three extracellular Ig-like domains (3). After binding IgG, CD64 interacts with an accessory chain known as the FcR common γ chain (FcRγ), which possesses an immunoreceptor tyrosine-based activation motif (ITAM) that is necessary for triggering cellular activation (2,3). Alternate Names AI323638; AV092959; CD64; Fc receptor, IgG, high affinity I; Fc-gamma RI; Fcg1; Fcgam; FcgammaRI; Fcgr1; FCGR1A; FcRI; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; IGGHAFC Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG