CST, 90704T, TOM22 (E3F4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TOMM22. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TOM22 (E3F4M) Rabbit Monoclonal Antibody (CST #90704) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TOM22 (E3F4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total TOM22 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TOM22 protein. Background Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1). TOM20 is localized in the outer mitochondrial membrane and initially recognizes precursors with a presequence to facilitate protein import across the outer mitochondrial membrane (2). In a sequential process, recognition of the presequence by TOM20 is followed by tethering of the presequence to the TOM40 protein complex for efficient protein import (3). Alternate Names 1C9-2; hTom22; Mitochondrial import receptor subunit TOM22 homolog; mitochondrial import receptor Tom22; MST065; MSTP065; TOM22; TOMM22; Translocase of outer membrane 22 kDa subunit homolog; translocase of outer mitochondrial membrane 22; translocase of outer mitochondrial membrane 22 homolog; translocase of outer mitochondrial membrane 22 homolog (yeast) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG