CST, 90709T, NOP2 (E9Z4D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NOP2. Validated for WB,IP,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, NOP2 (E9Z4D) Rabbit Monoclonal Antibody (CST #90709) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NOP2 (E9Z4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total NOP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu221 of human NOP2 protein. Background Chemical modifications of RNA regulate many cellular processes. One particular RNA modification, 5-methylcytosine (5-mC), regulates ribosome assembly, translation, and RNA stability (1). In eukaryotes, this modification is added to RNA by the DNA methyltransferase homologue 2 protein (DNMT2; also known as TRDMT1), and also by members of the NOL1/NOP2/SUN domain (NSUN) family of proteins. NSUN proteins are putative S-adenosylmethionine (SAM)-dependent methyltransferases that carry out their enzymatic activity by utilizing two cysteine residues in their active sites (2). There are currently seven known members of this family, consisting of NOP2 (NSUN1) and NSUN2-7. NOP2, also known as NSUN1, is an 89 kDa member of the NSUN protein family that specifically targets and methylates 28S rRNA (3). In humans, methylation of C4413 in the 28S rRNA by NOP2 is thought to increase stability of the ribosome. NOP2 is strongly overexpressed in many cancers, including colorectal and lung cancer, where it may contribute to tumorigenesis by recruiting telomerase to the cyclin D1 promoter and activating gene expression (4-6). NOP2 also interacts with BRD4 and RNA polymerase II, suggesting additional roles for NOP2 in regulating transcription (1,7). Alternate Names MGC117384; MGC149287; MGC149288; NOL1; NOL1/NOP2/Sun domain family, member 1; NOP120; NOP2; NOP2 nucleolar protein; NOP2 nucleolar protein homolog; NOP2 nucleolar protein homolog (yeast); NOP2/Sun domain family, member 1; NSUN1; Nucleolar protein 1; nucleolar protein 1, 120kDa; Nucleolar protein 2 homolog; p120; Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; Proliferating-cell nucleolar antigen p120; Proliferation-associated nucleolar protein p120; Putative ribosomal RNA methyltransferase NOP2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG