CST, 9081S, hnRNP K (D9A8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP K. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, hnRNP K (D9A8) Rabbit Monoclonal Antibody (CST #9081) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity hnRNP K (D9A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP K protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNP K protein. Background Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7), and signal transduction. Research studies have shown that cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer, and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11). Alternate Names AUKS; CSBP; dC-stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; Transformation up-regulated nuclear protein; transformation upregulated nuclear protein; TUNP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58-62 Source/Isotype: Rabbit IgG