CST, 90851S, SC-79

Chemical Modulators for studying in the research area. Product Usage Information SC-79 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 0.91 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect. Storage Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles . Background The small-molecule SC-79 activates cytosolic Akt (PKB, protein kinase B) and inhibits Akt translocation to the membrane. Binding of SC-79 to Akt promotes interaction between Akt and the protein kinase PDK1, leading to phosphorylation of Akt at Thr308 and Ser473 (1). In a mouse model of acute liver failure, SC-79 treatment protects hepatocytes from apoptosis by inhibiting Akt-mediated aggregation of Fas and by blocking recruitment of FADD and procaspase-8 into the death-inducing signaling complex (2). Likewise, SC-79 protects hepatocytes from tumor necrosis factor-α (TNF-α)-induced apoptosis and mice from induced liver injury and damage (3). In a rat model of alcohol-induced osteonecrosis, SC-79 exposure leads to activation of cytosolic Akt and attenuation of bone tissue morphological changes (4). SC-79 treatment of neuroblastoma cells and murine dopaminergic neurons leads to Akt activation and protection from hydrogen peroxide-induced apoptosis and necrosis (5).