CST, 9091T, LAMP1 (D2D11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP1. Validated for WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, LAMP1 (D2D11) Rabbit Monoclonal Antibody (CST #9091) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 52252 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LAMP1 (D2D11) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names CD107 antigen-like family member A; CD107a; LAMP-1; LAMP1; LAMPA; LGP120; lysosomal associated membrane protein 1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 42 (non-glycosylated), 90-120 (glycosylated) Source/Isotype: Rabbit IgG