CST, 90947T, STING (E9X7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STING. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, STING (E9X7F) Rabbit Monoclonal Antibody (CST #90947) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:800 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Flow Cytometry (Fixed/Permeabilized): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37230 . Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity STING (E9X7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total STING protein. This antibody lacks sensitivity in some low-expressing mouse cell lines by immunofluorescence. This lack of sensitivity has not been observed in human cell lines. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with human STING recombinant protein and reacts with an epitope near the carboxy terminus. Background Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). Alternate Names endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; SAVI; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; STING; STING-beta; STING1; TM173; TMEM173; Transmembrane protein 173 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG