CST, 91025S, Miro2 (F3N5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MIRO2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Miro2 (F3N5C) Rabbit Monoclonal Antibody (CST #91025) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Miro2 (F3N5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Miro2 protein. This antibody does not cross-react with Miro1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro176 of human Miro2 protein. Background Mitochondrial Rho GTPase 1 (Miro1, RHOT1) and mitochondrial Rho GTPase 2 (Miro2, RHOT2) are atypical Ras GTPase proteins that localize to the outer mitochondrial membrane (1,2). These evolutionarily conserved proteins contain GTP-binding domains and a pair of calcium-binding EF hand domains (1,2). Research studies indicate that Miro1 and Miro2 function in the axonal transport of mitochondria in neurons (2). Both Miro proteins play an essential role in mitochondrial trafficking by attaching mitochondria to essential motor and adaptor proteins (3). Miro GTPase proteins that are anchored to the outer mitochondrial membrane interact with kinesin-binding proteins TRAK1 and TRAK2 to provide a link between mitochondria to microtubules (4). Increased synaptic calcium levels appear to inhibit mitochondrial trafficking mediated by Miro, suggesting a role for the EF hand as a calcium sensor (5). Alternate Names ARHT2; C16orf39; hMiro-2; MIRO-2; MIRO2; mitochondrial Rho (MIRO) GTPase 2; mitochondrial Rho 2; Mitochondrial Rho GTPase 2; ras homolog family member T2; Ras homolog gene family member T2; ras homolog gene family, member T2; RASL; RHOT2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG