CST, 91137S, Pannexin-1 (D9M1C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PANX1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pannexin-1 (D9M1C) Rabbit Monoclonal Antibody (CST #91137) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pannexin-1 (D9M1C) Rabbit Monoclonal Antibody recognizes endogenous levels of total pannexin-1 protein. This antibody detects an amino-terminal pannexin-1 fragment produced by caspase cleavage. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human pannexin-1 protein. Background The pannexin family (pannexin-1, -2, and -3; PANX1-3) of gap junction proteins has homology to the invertebrate innexins and display distinct expression patterns (1). Pannexin-1 is widely expressed, with highest expression in the heart, brain, skeletal muscle, testis, and ovary (1,2). Pannexin-2 is predominately expressed in the brain (1,2) and pannexin-3 is found within the skin and connective tissues (1,3). Connexin family gap junction proteins form hemichannels that align adjacent cells, creating functional intercellular channels that are permeable to ions and small molecules. In contrast, pannexin proteins may not function as gap junction proteins since pannexins on adjacent cells may not align to form complete channels (3). These pannexin "hemichannels" may play a role in inflammation, apoptosis, and neuronal signaling by allowing permeability of ions, ATP, and potentially other small molecules into the extracellular space (4-6). Pannexin-1 can be activated by effector caspases (caspase-3 and -7), which leads to release of signal molecules that promote phagocytosis of apoptotic cells (7). Alternate Names innexin; MGC21309; MRS1; OOMD7; pannexin 1; Pannexin-1; PANX1; PX1; UNQ2529 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45-55, 19 Source/Isotype: Rabbit IgG