CST, 91138T, Lamellipodin (D8A2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lamellipodin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Lamellipodin (D8A2K) Rabbit Monoclonal Antibody (CST #91138) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Lamellipodin (D8A2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total lamellipodin protein. Based on the amino acid sequence of the immunogenic peptide, this antibody is expected to detect the canonical isoform (RMO1-RAPH1) only. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro990 of human lamellipodin protein. Background Regulation of actin dynamics at the leading edge of the cell drives cell adhesion, migration, and invasion. These processes are crucial for normal development, immune cell function, angiogenesis and cell division, and are dysregulated in diseases such as metastatic cancer (1, 2). Lamellipodin (Lpd) is a member of the Mig-10/RIAM/lamellipodin (MRL) family of adaptor proteins. Lamellipodin regulates the formation of lamellipodia at the leading edge through its interaction with and recruitment of actin-modulating proteins such as Ena/VASP (3) and the Scar/WAVE complex (4). Lamellipodin and Mena cooperate with endophilin to regulate EGFR endocytosis (5). MRL proteins can also form complexes with talin and activated integrins, guiding the formation of cell protrusions and the direction of cell migration (6). Alternate Names ALS2CR18; ALS2CR9; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 18; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 9; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; KIAA1681; Lamellipodin; LPD; PREL-2; PREL2; proline rich EVH1 ligand 2; Proline-rich EVH1 ligand 2; Protein RMO1; RalGDS/AF-6; RAPH1; Ras association (RalGDS/AF-6) and pleckstrin homology domains 1; Ras-associated and pleckstrin homology domains-containing protein 1; RMO1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 195 Source/Isotype: Rabbit IgG