CST, 9115S, Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRD1. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (CST #9115) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 89036 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody detects endogenous levels of total Blimp-1/PRDI-BF1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1 isoform 2. Background Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T cells (4,5), suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13). Alternate Names B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP-1; BLIMP1; MGC118922; MGC118923; MGC118924; MGC118925; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR-domain zinc finger protein 1; PR/SET domain 1; PRDI-BF1; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 95-100 Source/Isotype: Rabbit IgG