CST, 9119S, Maspin (L250) Antibody

Polyclonal Antibody for studying Maspin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Maspin (L250) Antibody (CST #9119) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Maspin (L250) Antibody detects endogenous levels of total maspin protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu250 of human maspin. Antibodies were purified by peptide affinity chromatography. Background Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9). Alternate Names Maspin; Peptidase inhibitor 5; PI-5; PI5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5; SPB5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit