CST, 91213SF, CD20 (Rituximab Biosimilar) Human Monoclonal Antibody

Monoclonal Antibody for studying CD20. Validated for Neutralizing. Highly specific and rigorously validated in-house, CD20 (Rituximab Biosimilar) Human Monoclonal Antibody (CST #91213) is ready to ship. Product Usage Information This product is intended for research use only (RUO). Optimal dilutions/concentrations should be determined by the end user. Formulation Supplied in 1X PBS, BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD20 (Rituximab Biosimilar) Human Monoclonal Antibody was confirmed to bind to its intended target protein CD20 using flow cytometry. Species Reactivity: Human Source / Purification CD20 (Rituximab Biosimilar) Human mAb is produced at Cell Signaling Technology. Rituximab is an antibody directed against the extracellular region of human CD20. Background B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6). Alternate Names B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp35; CD20; CD20 antigen; CD20 receptor; CVID5; LEU-16; Leukocyte surface antigen Leu-16; membrane spanning 4-domains A1; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969; MS4A1; MS4A2; S7 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Human IgG1 kappa