CST, 91327T, hnRNP C1/C2 (D6S3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP C1/C2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, hnRNP C1/C2 (D6S3N) Rabbit Monoclonal Antibody (CST #91327) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity hnRNP C1/C2 (D6S3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP C1/C2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile217 of human hnRNP C1/C2 protein. Background Heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2) has multiple biological functions including transcriptional regulation, DNA repair, and RNA processing. hnRNP C1/C2 acts as a ‘molecular ruler' in the mRNA processing pathway, committing nascent transcripts from the chromatin template to the mRNA export pathway once the nascent transcript becomes longer than 200-300 nucleotides (1). hnRNP C1/C2 associates with SWI/SNF and NurD family members to form the locus control region (LCR)-associated remodeling complex (LARC), which binds to β-globin gene promoter to prevent transcriptional silencing. Studies indicate that without hnRNP C1/C2, LARC does not associate with its target DNA sequence (2,3). hnRNP C1/C2 and other hnRNP family members interact with DNA damage response (DDR) proteins (4). hnRNP proteins regulate double stranded break (DSB) repair by promoting either homologous recombination (HR) or non-homologous end joining (NHEJ) (4). hnRNP C1/C2 downregulates the expression of miR-21, which leads to the increased expression of programmed cell death 4 (PDCD4) protein in glioblastoma multiforme (GBM) (5). Research studies have shown that silencing of hnRNP C1/C2 renders GBM cells more susceptible to apoptosis (5). Alternate Names C1; C2; heterogeneous nuclear ribonucleoprotein C; heterogeneous nuclear ribonucleoprotein C (C1/C2); Heterogeneous nuclear ribonucleoproteins C1/C2; HNRNP; hnRNP C1/C2; HNRNPC; HNRPC; MGC104306; MGC105117; MGC117353; MGC131677; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein; SNRPC Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG