CST, 9139T, Stat3 (124H6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying STAT3. Validated for WB,WB,WB,IP,IHC,IF,F,ChIP,C&R. Available in 3 sizes. Highly specific and rigorously validated in-house, Stat3 (124H6) Mouse Monoclonal Antibody (CST #9139) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 IHC Leica Bond: 1:300 - 1:1200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Chromatin IP: 1:100 CUT&RUN: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83541 . Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, IHC Leica Bond, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN Specificity / Sensitivity Stat3 (124H6) Mouse Monoclonal Antibody detects endogenous levels of total Stat3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide centered around amino acid Gln692 of human Stat3. Background The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). Alternate Names Acute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 79, 86 Source/Isotype: Mouse IgG2a kappa