CST, 91462S, TRIM21 (F2E4V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM21 mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM21 (F2E4V) Rabbit Monoclonal Antibody (CST #91462) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRIM21 (F2E4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM21 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln320 of mouse TRIM21 protein. Background Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons (IFNs) and acts as a cytosolic IgG receptor (1,2). TRIM21 is an activator of the innate immune system and targets antibody-bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol (3,4). Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced IFN production through the polyubiquitin-mediated degradation of multiple targets, including IRF3, IRF7, and DDX41 (5-7). Alternate Names 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; E3 ubiquitin-protein ligase TRIM21; RING-type E3 ubiquitin transferase TRIM21; Ro(SS-A); Ro52; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; SS-A; Ssa1; Trim21; tripartite motif protein 21; tripartite motif-containing 21; Tripartite motif-containing protein 21 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG