CST, 9147T, MEK2 (13E3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEK2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MEK2 (13E3) Rabbit Monoclonal Antibody (CST #9147) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MEK2 (13E3) Rabbit Monoclonal Antibody detects endogenous levels of total MEK2 protein. This antibody does not cross-react with MEK1 and other MAP kinase kinases. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human MEK2. Â Background MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. Alternate Names CFC4; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; FLJ26075; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2; MAPKK2; MEK 2; MEK2; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK2; MP2K2; PRKMK2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG