CST, 91541S, TUFT1 (E5W5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TUFT1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, TUFT1 (E5W5I) Rabbit Monoclonal Antibody (CST #91541) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TUFT1 (E5W5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TUFT1 protein. This antibody detects a 28 kDa protein of unknown origin by western blot. Reactivity for immunoflourescence is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TUFT1 protein. Background TUFT1 (Tuftelin 1) is a secreted protein that is primarily expressed in the dental pulp and ameloblasts, the cells responsible for producing and secreting enamel matrix proteins during tooth development. The protein is involved in the formation of enamel crystals and plays a critical role both in tooth development and maintenance (1). Some studies have suggested that TUFT1 may play a potential role in disease. TUFT1 was shown to promote metastasis in pancreatic cancer by promoting EMT (2,3), and unregulated TUFT1 expression was shown to be associated with metastatic potential and a poor prognosis in breast cancer (4). TUFT1 has also been reported to play pro-tumorigenic roles in cancer types such as renal and gastric cancer (5,6). Alternate Names TUFT1; Tuftelin; tuftelin 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG