CST, 9163S, Phospho-Merlin (Ser518) Antibody

Polyclonal Antibody for studying Merlin (Ser518) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Merlin (Ser518) Antibody (CST #9163) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Merlin (Ser518) Antibody detects endogenous levels of merlin only when phosphorylated at Ser518. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser518 of human Merlin protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Neurofibromatosis 2 (NF2) is an autosomal dominant, inherited disorder characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors found in the general population are caused by inactivation of the tumor suppressor gene. Merlin (moesin, ezrin, and radixin-like protein) is the gene product, displaying striking similarity to ezrin, radixin, and moesin (ERM) proteins. Regulation of merlin (also called schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4). Alternate Names ACN; BANF; MERL; Merlin; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); Neurofibromin-2; NF2; SCH; Schwannomerlin; Schwannomin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit