CST, 91684S, MED13 (E4E9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Trap240/MED13. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MED13 (E4E9H) Rabbit Monoclonal Antibody (CST #91684) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MED13 (E4E9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total MED13 protein. This antibody recognizes a 50 kDa protein of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1592 of human MED13 protein. Background The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10). Alternate Names Activator-recruited cofactor 250 kDa component; ARC250; DRIP250; HSPC221; KIAA0593; MED13; Mediator complex subunit 13; Mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; MRD61; THRAP1; thyroid hormone receptor associated protein 1; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; TRAP240; Vitamin D3 receptor-interacting protein complex component DRIP250 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG