CST, 91771S, Periostin Antibody

Polyclonal Antibody for studying periostin. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Periostin Antibody (CST #91771) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Periostin Antibody recognizes endogenous levels of total periostin protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser395 of human periostin protein. Antibodies are purified by peptide affinity chromatography. Background Periostin (POSTN, OSF2) is a secretory matricellular protein that belongs to the fasciclin family. The protein has multiple functional domains that interact with different extracellular matrix (ECM) components (1-2). The N-terminal EMI domain of periostin interacts with fibronectin as well as collagens and lysyl oxidase. The middle part of the protein consists of four FAS 1 (fasciclin) domains which are responsible for the protein association with tenascin-C and CCN3. The C-terminal CTD domain assumes an inhibitory effect on FAS1 domain function. Lastly, the C-terminal HBD domain mediates proteoglycan association (1-2). Periostin has been shown to mediate important cellular processes such as ECM stemness, mechanical stress response, collagen cross-linking and fibrogenesis, cell proliferation, survival, and adhesion (1-3). It is an important modulator in multiple disease developments and has been frequently used as a biomarker for cancer, fibrosis, inflammation, and tissue wound healing (4-7). Alternate Names MGC119510; MGC119511; OSF-2; OSF2; osteoblast specific factor 2 (fasciclin I-like); Osteoblast-specific factor 2; PDLPOSTN; periodontal ligament-specific periostin; Periostin; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; periostin, osteoblast specific factor; PN; POSTN; RP11-412K4.1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit