CST, 9177S, Phospho-Stat1 (Ser727) Antibody

Polyclonal Antibody for studying Stat1 (Ser727) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Chromatin IP. Highly specific and rigorously validated in-house, Phospho-Stat1 (Ser727) Antibody (CST #9177) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity Phospho-Stat1 (Ser727) Antibody detects endogenous levels of Stat1α only when phosphorylated at Ser727. This site is deleted in Stat1β. This antibody does not significantly cross-react with the corresponding phosphorylated residues of other Stat proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography. Background The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. Alternate Names CANDF7; DKFZp686B04100; IMD31A; IMD31B; IMD31C; ISGF-3; signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91; Transcription factor ISGF-3 components p91/p84 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 91 SOURCE: Rabbit