CST, 91788S, PI31/PSMF1 Antibody

Polyclonal Antibody for studying PSMF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, PI31/PSMF1 Antibody (CST #91788) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PI31/PSMF1 Antibody recognizes endogenous levels of total PI31/PSMF1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro245 of human PI31/PSMF1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Proteasome-binding protein PI31 (Proteasomal Inhibitor of 31kD) is encoded by the gene and regulates proteasome function in the cell, which is essential for cell function and survival. Regulated degradation of intracellular proteins is mediated by the ubiquitin-proteasome system (UPS) by a multi-step process (1,2). First, protein to be degraded is tagged with a chain of ubiquitin molecules. Ubiquitinated proteins are then cleaved into small peptides by the 26S proteasome. Proteasome activity by the UPS is regulated at additional levels, including transcriptional expression of proteasomal proteins, posttranslational modification, and by various regulatory proteins. PI31/PSMF1 is a structurally conserved proteasome regulator that binds to the 20S proteasome and functions to inhibit 20S proteasome hydrolysis of small peptides (3,4). Unexpectedly, PI31/PSMF1 promotes UPS-mediated protein breakdown , suggesting additional proteasome regulatory functions beyond inhibition of the 20S proteasome (5). PI31/PSMF1 also serves as an adaptor protein to transport proteasomes via cellular protein motors (6,7). This process may be particularly important in neurons where local proteasomal degradation at distal tips of neurons may play a critical role in the maintenance of neuronal architecture, circuitry, and function. Alternate Names hPI31; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor hP131 subunit; Proteasome inhibitor PI31 subunit; proteasome inhibitor subunit 1; PSMF1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28, 29 SOURCE: Rabbit