CST, 9179S, FAAH1 Antibody

Polyclonal Antibody for studying FAAH. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, FAAH1 Antibody (CST #9179) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FAAH1 Antibody detects endogenous levels of total FAAH1 protein. Species Reactivity: Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of rat FAAH1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1). Alternate Names Anandamide amidohydrolase 1; FAAH; FAAH-1; FAAH1; fatty acid amide hydrolase; Fatty acid ester hydrolase; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase 1; PSAB Specification REACTIVITY: R SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit