CST, 9185S, Cleaved PARP (Asp214) (D64E10) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying PARP1 (Asp214, 89 kD) cleaved. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cleaved PARP (Asp214) (D64E10) Rabbit Monoclonal Antibody (Biotinylated) (CST #9185) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved PARP (Asp214) (D64E10) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full length PARP1 or other PARP isoforms. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 in human PARP protein. Background PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases (2,3) and is one of the main cleavage targets of caspase-3 (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Alternate Names ADP-ribosyltransferase (NAD+); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 89 Source/Isotype: Rabbit IgG