CST, 9186S, Phospho-Elk-1 (Ser383) (2B1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Elk-1 (Ser383) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Elk-1 (Ser383) (2B1) Mouse Monoclonal Antibody (CST #9186) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Elk-1 (Ser383) (2B1) Mouse Monoclonal Antibody detects less than 5 ng of phosphorylated Elk-1, and will not react with up to 1 µg of nonphosphorylated Elk-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1. Background The ETS transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation . Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). Alternate Names Elk-1; ELK1; ELK1, ETS transcription factor; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS transcription factor ELK1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene Specification REACTIVITY: H SENSITIVITY: Recombinant protein MW (kDa): 47 Source/Isotype: Mouse IgG1