CST, 91882S, CD86 (E2G8P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD86. Validated for WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD86 (E2G8P) Rabbit Monoclonal Antibody (CST #91882) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76755 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD86 (E2G8P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD86 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro239 of human CD86 protein. Background CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9). Alternate Names Activation B7-2 antigen; B-lymphocyte activation antigen B7-2; B7-2; B7-2 antigen; B7.2; B70; BU63; CD28 antigen ligand 2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 molecule; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60-85 Source/Isotype: Rabbit IgG