CST, 91939S, DAP12 (D7G1X) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Dap12. Validated for Western Blotting. Highly specific and rigorously validated in-house, DAP12 (D7G1X) Rabbit Monoclonal Antibody (Biotinylated) (CST #91939) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DAP12 (D7G1X) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total DAP12 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DAP12 protein. Background DNAX-activating protein 12 (DAP12, TYROBP) is a signaling adaptor for several pathogen receptors expressed by cells of the innate immune system (1). The DAP12 protein structure consists of a short extracellular domain, a transmembrane domain, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM) (2). DAP12 protein is expressed by hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, mast cells, basophils, eosinophils, neutrophils, and some γδ T cells and NKT cells (1). DAP12 exists as a homodimer that associates with a variety of receptors involved in pathogen detection, including the KIR family of NK cell receptors (2,3). Ligand binding by DAP12-associated receptors results in phosphorylation of tyrosine residues within the DAP12 ITAM by Src family kinases and leads to activation of Syk or Zap-70 and downstream signaling responses (2). Alternate Names DAP12; DNAX adaptor protein 12; DNAX-activation protein 12; KAR-associated protein; KARAP; killer activating receptor associated protein; Killer-activating receptor-associated protein; PLOSL; PLOSL1; polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; transmembrane immune signaling adaptor TYROBP; TYOBP; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 10, 12 Source/Isotype: Rabbit IgG