CST, 91968S, Phospho-IRF-3 (Ser386) (E7J8G) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying IRF-3 (Ser386) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-IRF-3 (Ser386) (E7J8G) Rabbit Monoclonal Antibody (PE Conjugate) (CST #91968) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-IRF-3 (Ser386) (E7J8G) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser386. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser386 of human IRF-3 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Alternate Names IIAE7; Interferon regulatory factor 3; IRF-3; IRF3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG