CST, 9197S, CREB (48H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CREB. Validated for WB,IP,IHC,IF,IF,F,ChIP,C&R,C&T. Available in 3 sizes. Highly specific and rigorously validated in-house, CREB (48H2) Rabbit Monoclonal Antibody (CST #9197) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:250 Immunohistochemistry (Paraffin): 1:6400 - 1:25600 Immunofluorescence (Frozen): 1:800 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Chromatin IP: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 56820 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity CREB (48H2) Rabbit Monoclonal Antibody detects endogenous levels of total CREB-1 protein. This antibody does not cross-react with other ATF/CREB family members. Non-specific staining of components along the retinotectal pathway was observed by immunofluorescence in fixed frozen mouse tissue. Non-specific signal is observed in formaldehyde fixed frozen mouse retina by immunofluorescence. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CREB-1 protein. The epitope has been mapped to residues surrounding Glu21. Background CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca , and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). Alternate Names active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG