CST, 91982S, LbCpf1/Cas12a (Strain ND2006) (2D5-6G11) Mouse Monoclonal Antibody

Monoclonal Antibody for studying LbCpf1 (Strain ND2006). Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, LbCpf1/Cas12a (Strain ND2006) (2D5-6G11) Mouse Monoclonal Antibody (CST #91982) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LbCpf1/Cas12a (Strain Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of LbCpf1/Cas12a (Strain ) protein. Background CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from and ) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).~LbCpf1 (Strain )/Cas12a is a Cpf1/Cas12a enzyme derived from (5,6). Alternate Names Cpf1; LbCpf1 Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 143 Source/Isotype: Mouse IgG1