CST, 92106W2, GATA-4 (D3A3M) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying GATA4. Highly specific and rigorously validated in-house, GATA-4 (D3A3M) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 555 Conjugate) (CST #92106) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity GATA-4 (D3A3M) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-4 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-4 is crucial for cardiomyocyte differentiation (4), and not surprisingly, mutations in the gene are implicated in many cardiac diseases, such as tetralogy of Fallot (5), familial and sporadic dilated cardiomyopathy (6,7), and atrial septal defect (8). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (9). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (10). GATA-4 is also a required factor for bone mineralization in osteoblast progenitor cells, where it suppresses TGF-β and activates BMP signaling (11). Research studies have uncovered crosstalk among the transcription factors GATA-4, GATA-6, and KLF5 that promotes gastric cancer development (12). Additional studies describe the role of histone H3K9 hyperacetylation on the GATA-4 promoter in fetal mice that have been exposed to ethanol (13). Alternate Names ASD2; GATA binding protein 4; GATA-binding factor 4; GATA-binding protein 4; GATA4; MGC126629; TACHD; TOF; Transcription factor GATA-4; VSD1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG