CST, 92108S, PELO (F3V8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PELO. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PELO (F3V8O) Rabbit Monoclonal Antibody (CST #92108) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PELO (F3V8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total PELO protein. This antibody detects a 120 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His244 of human PELO protein. Background PELO, also known as Pelota or Dom34, along with its binding partner HBS1, is involved in several key mRNA quality control mechanisms (1). The PELO-HBS1 complex utilizes the ATPase ABCE1 as an ATPase to cause dissociation of stalled elongation complexes (3,4). PELO-HBS1 can cause mRNA degradation through two main pathways: the nonstop mRNA degradation pathway, where the mRNA lacks a stop codon, and the no-go mRNA degradation pathway, where the ribosome stalls due to secondary structures or damaged nucleotides (2-5). PELO has been shown to play a multifaceted role in viral infection, detecting single-stranded viral RNAs and targeting them for degradation, while also facilitating RNA turnover for more efficient viral replication (6,7). PELO has been implicated in protection from age-related disease phenotypes (8). Alternate Names CGI-17; PELO; pelota homolog; pelota homolog (Drosophila); pelota mRNA surveillance and ribosome rescue factor; PRO1770; Protein pelota homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG