CST, 9212S, p38 MAPK Antibody

Polyclonal Antibody for studying p38-alpha MAPK/p38-gamma MAPK/p38-beta MAPK. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, p38 MAPK Antibody (CST #9212) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:150 - 1:600 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p38 MAPK Antibody detects endogenous levels of total p38α, -β or -γ MAPK protein. This antibody does not recognize p38δ, JNK/SAPK or p44/42 MAPK. Species Reactivity: Human, Mouse, Rat, Monkey, Guinea Pig Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro350 of human p38 MAPK protein. Antibodies are purified by protein A and peptide affinity chromatography. Background p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Alternate Names CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; ERK-6; ERK3; ERK6; EXIP; Extracellular signal-regulated kinase 6; MAP kinase 11; MAP kinase 12; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 gamma; MAPK 11; MAPK 12; MAPK 14; MAPK11; MAPK12; MAPK14; MAX-interacting protein 2; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; Mitogen-activated protein kinase p38 gamma; mitogen-activated protein kinase p38-2; MK11; MK12; MK14; MXI2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38-2; p38-alpha; P38A; p38ALPHA; p38alpha Exip; p38b; p38Beta; P38BETA2; P38G; P38GAMMA; PRKM11; PRKM12; PRKM14; PRKM15; RK; SAPK-3; SAPK2; SAPK2A; SAPK2B; SAPK3; Stress-activated protein kinase 2; stress-activated protein kinase 2A; Stress-activated protein kinase 2b; Stress-activated protein kinase 3; stress-activated protein kinase-2; stress-activated protein kinase-2b Specification REACTIVITY: H M R Mk GP SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit