CST, 92145S, TTF-I Antibody

Polyclonal Antibody for studying TTF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TTF-I Antibody (CST #92145) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TTF-I Antibody recognizes endogenous levels of total TTF-I protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His34 of human TTF-I protein. Antibodies are purified by peptide affinity chromatography. Background Transcription termination factor I (TTF-I) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF-I binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3' ends of the genes. Binding of TTF-I to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC (1-5). TTF-I also has an activating function by binding an upstream terminator element to induce chromatin remodeling (6). TTF-I localization can be controlled by key tumor suppressor ARF, which binds to the TTF-I nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis (7). Furthermore, TTF-I can be ubiquitinylated by MDM2, which targets it for degradation (8). TTF-I has also been identified to play a role in HIV-1 replication (9). Alternate Names RNA polymerase I termination factor; Transcription termination factor 1; Transcription termination factor I; transcription termination factor, RNA polymerase I; TTF-1; TTF-I; TTF1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 135 SOURCE: Rabbit