CST, 92189S, Lu/BCAM Antibody

Polyclonal Antibody for studying BCAM. Validated for Western Blotting. Highly specific and rigorously validated in-house, Lu/BCAM Antibody (CST #92189) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Lu/BCAM Antibody recognizes endogenous levels of total Lu/BCAM protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala587 of human Lu/BCAM protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Lu/BCAM (CD239) is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily (1). The protein mediates cell adhesion by binding to basal membrane laminin α5, and cell surface integrin α4β1 (2,3). The interaction between Lu/BCAM and its ligands can be activated via phosphorylation in its cytoplasmic tail by either PKA or Akt signaling (4,5). In sickle cell disease, Lu/BCAM was reported to promote abnormal red blood cell adhesion to endothelium, and thus may contribute to vaso-occlusive crisis (6). Overexpression of Lu/BCAM has also been reported in breast cancer, skin cancer, colon cancer, and ovarian cancer (7, 8). Antibody-drug conjugates targeting Lu/BCAM on tumor cells surface has been proposed as a potential approach for cancer therapy (8). Alternate Names antigen identified by monoclonal antibody F8; AU; Auberger B antigen; B-CAM cell surface glycoprotein; B-cell adhesion molecule; Basal cell adhesion molecule; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); BCAM; CD239; F8/G253 antigen; glycoprotein 95kDa; LU; Lutheran antigen; Lutheran blood group glycoprotein; Lutheran blood group variant LUGA; MSK19 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85, 88 SOURCE: Rabbit