CST, 92190S, SP3 (E6Q9Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SP3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SP3 (E6Q9Q) Rabbit Monoclonal Antibody (CST #92190) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SP3 (E6Q9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total SP3 protein. The antibody shows weak cross-reactivity with an 85 kDa protein of unknown identity in some cell extracts. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SP3 protein. This antibody recognizes the large isoforms of SP3 (e.g., L-SP3), but does not recognize the short isoforms generated by alternative splicing or initiation. Background SP3 (Specificity protein 3, Transcription factor Sp3) is a member of the Specificity Protein/Krüppel-like Factor (SP/KLF) family of DNA-binding transcription factors, which play a variety of important roles in cell function and homeostasis (1). There are nine known SP transcription factors (SP1-9); each contains a C2H2 zinc finger domain responsible for high affinity binding to GC-rich motifs that enables the activation or repression of target gene expression (2). SP1-4 contain a functional transactivation (TAD) in their amino terminus, which is notably absent from SP5-9. Despite nearly ubiquitous expression of SP transcription factors across most normal cells and tissues, research studies have identified links between expression levels and disease. It was shown, for example, that combined overexpression of v-Myc and SP1/SP3 led to oncogenic transformation of fibroblasts (3). Increased levels of SP transcription factors have likewise been reported in various tumor cells (2), while SP1, SP3, and SP4 were all reported to bind the VEGFR promoter and increase VEGF receptor expression in pancreatic cancer cells (4). The latter observation suggests that SP transcription factors may promote tumor cell growth through multiple mechanisms, including enhancing tumor angiogenesis, providing a potential avenue to therapeutic intervention. Alternate Names DKFZp686O1631; GC-binding transcription factor Sp3; SP3; Sp3 transcription factor; specificity protein 3; SPR-2; SPR2; Transcription factor Sp3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 115, 120 Source/Isotype: Rabbit IgG