CST, 92192S, SCOT1/OXCT1 (F1O1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCOT1/OXCT1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SCOT1/OXCT1 (F1O1D) Rabbit Monoclonal Antibody (CST #92192) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SCOT1/OXCT1 (F1O1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SCOT1/OXCT1 protein. This antibody does not cross-react with SCOT2/OXCT2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val180 of human SCOT1/OXCT1 protein. Background Mitochondrial succinyl-CoA:3-ketoacid CoA transferase 1 (SCOT1), also known as 3-oxoacid CoA-transferase 1 (OXCT1), catalyzes the rate-limiting step of ketone body oxidation by transferring CoA from succinyl-CoA to acetoacetate to form acetoacetyl-CoA (1,2). Studies show that SCOT1/OXCT1 expression and activity in skeletal muscle increase in obesity. Obesity-induced hyperglycemia is alleviated in skeletal muscle-specific knockout mice (1). In addition, SCOT1/OXCT1 functions as a lysine succinyltransferase. Succinylation of serine beta-lactamase-like protein (LACTB) by SCOT1/OXCT1 at K284 suppresses LACTB proteolytic activity, leading to hepatocellular carcinoma (HCC) progression (2). Furthermore, studies show that SCOT1/OXCT1 succinylation and activation mediated by SUCLA2 promote ketolysis and HCC cell proliferation in mice (3). Alternate Names 3-oxoacid CoA transferase; 3-oxoacid CoA transferase 1; 3-oxoacid CoA-transferase 1; 3-oxoacid-CoA transferase 1; epididymis secretory sperm binding protein; OXCT; OXCT1; SCOT; SCOT-s; SCOT1; somatic-type succinyl CoA:3-oxoacid CoA-transferase; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; Somatic-type succinyl-CoA:3-oxoacid-CoA-transferase; succinyl CoA:3-oxoacid CoA transferase; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; succinyl-CoA:3-ketoacid-CoA transferase; Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG