CST, 92215S, Tryptase (E5F3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tryptase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Tryptase (E5F3Q) Rabbit Monoclonal Antibody (CST #92215) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tryptase (E5F3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tryptase protein. This antibody does not cross-react with murine Tryptase proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala120 of human Tryptase protein. Background Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells (1). Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent) (2). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin (3-5). Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides (6), calcitonin gene-related peptides (7), fibronectins (8), fibrinogens (3), and kininogens (9). Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts (10-13). Alternate Names epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; TPS1; TPS2; TPSAB1; TPSB1; TPSB2; TRYA1; TRYB1; tryptase alpha II; Tryptase alpha-1; tryptase alpha/beta 1; Tryptase alpha/beta-1; tryptase beta 1; tryptase beta I; tryptase beta-1; Tryptase beta-2; tryptase I; Tryptase II; Tryptase-1; Tryptase-2; tryptase-I; tryptase-III Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25-40 Source/Isotype: Rabbit IgG