CST, 9228S, p38 alpha MAPK (L53F8) Mouse Monoclonal Antibody

Monoclonal Antibody for studying p38-alpha MAPK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, p38 alpha MAPK (L53F8) Mouse Monoclonal Antibody (CST #9228) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity p38 alpha MAPK (L53F8) Mouse Monoclonal Antibody detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38. Species Reactivity: Human, Mouse, Rat, Monkey, Pig, S. cerevisiae Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK protein. Background p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Alternate Names CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; EXIP; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14; MXI2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38-alpha; P38A; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK2A; stress-activated protein kinase 2A Specification REACTIVITY: H M R Mk Pg Sc SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Mouse IgG1