CST, 92295S, KCNK6 Antibody

Polyclonal Antibody for studying KCNK6. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, KCNK6 Antibody (CST #92295) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KCNK6 Antibody recognizes endogenous levels of total KCNK6 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KCNK6 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Potassium channels are protein complexes that form potassium ion (K )-selective pores allowing the passive transport of K through biological membranes. They regulate K homeostasis and cell volume, and also have physiological functions involved in neurotransmitter and hormone secretion and muscular and neuronal excitability. Many potassium channel proteins have been identified and classified into different groups, mainly based on their membrane topology. The KCNK subfamily (Potassium channel subfamily K) is characterized by the presence of four transmembrane segments (4TMS) and the two-pore (2P) domain (4TMS/2P). These two-pore domain K (K2p) channels are considered as leaky channels that maintain background K currents to stabilize the negative membrane potential and counterbalance depolarization (1-4). KCNK6, also known as TWIK-2, is expressed in all human tissues examined except the skeletal muscle (5). A recent study identified KCNK6 as the K channel mediating K efflux across the plasma membrane that is essential for NLRP3 inflammasome activation (6). Alternate Names FLJ12282; Inward rectifying potassium channel protein TWIK-2; K2p6.1; K2P6.1 potassium channel; KCNK6; KCNK8; Potassium channel subfamily K member 6; potassium channel, subfamily K, member 6; potassium channel, two pore domain subfamily K, member 6; potassium two pore domain channel subfamily K member 6; TOSS; TWIK-2; TWIK-originated similarity sequence; TWIK-originated sodium similarity sequence; TWIK2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 35, 70 SOURCE: Rabbit